Gene technology Lab Report Example | Topics and Well Written Essays - 750 words

Gene technology - Lab Report Example The plasmid is 3.5 kb in size (Fig.5). It has two ECoRI restriction sites, hence should break any recombinant DNA into two fragments, i.e. ~3.5 kb and the inserted DNA. In our experiment – There are three fragments of DNA which shows that the inserted PCR product must have one ECoRI site. The ECoRI site in the PCR product is placed almost in the middle breaking it into two fragments, viz. 600 bp and 800 bp (Fig. 6). Interpretation - It appears that the insert has two restriction sites for NCoI (Fig 7). But position of the sites would result in a DNA fragment smaller than 1.5 kb (the total size of the insert), the size discrepancy is difficult to explain. Interpretation – This result is totally unexpected! The bands corresponding to both the restriction enzymes, i.e. at 1.0 kb, 2.5 kb, 3.0 kb are there but in addition there are three other intermediate size DNA fragments. The bands appear to be intermediates produced due to impartial digestion of the recombinant by the two restriction enzymes. Reason – Concentration of the enzymes was less, time of incubation is less than optimum or incubation conditions were inadequate. Sufficient time should be given for the restriction enzymes to act on all the sites. ii. NcoI digestion (Lane 4) – the restriction sites appear to be different than mine (Fig 4a). The NcoI sites appear to be closer in the 16S rDNA extracted by this student and give rise to a DNA fragment of 600 bp. Janda M. and Abbott S.L. (2007). 16S rRNA Gene Sequencing for Bacterial Identification in the Diagnostic Laboratory: Pluses, Perils, and Pitfalls. J. Clin Microbiol. 2007 September; 45(9): 2761–2764. Khare N., Sharma D., Somashekar U., Prakash A., Prakash S., Mendki M.J. and Anvikar A. (2008). Detection of bacterial DNA in cholesterol gall stones. The Internet Journal of Surgery 16 (2). Available from

Discuss what funerary art and architecture reveal about Roman social Research Paper

Discuss what funerary art and architecture reveal about Roman social issues - Research Paper Example Other pieces include symbols of a particular god or goddess who may have been something like patron saints for Romans who were poly-deists. Just like funerals nowadays have evolved to include slide shows of the deceased’s life and symbols of his/her accomplishments, Roman funerary art served to honor the person who had passed from one life to the next, but it also represents what the Romans valued: wealth, status, family, and the favor of the gods. Some of the most historically revealing funerary pieces represent the wealthy Romans, who had paintings, busts and statues of themselves and their families made in their likenesses. Some were placed at their tombs. Others were kept in the doma, the home, in a special place devoted just for the worship of deities and the commemoration of those who had passed. These paintings and sculptures looked amazingly like the person. At least they are less idealized than the commemorative pieces done for the lower ranked Romans. Often the pieces done for the wealthier Romans were commissioned at or near the time of death, or so it is presumed, because modern day methods of determining this say that the statues and busts resemble the person at the time of death more than in life. Consider the funerary portraiture from Roman Egypt. â€Å"Apparently these portraits were not made for display during the sitter’s life; comparison of the images with CAT scans of the remains inside these mummie s makes it clear that the portraits represent the deceased at the age of death† (Daily Life in Ancient Rome p. 145). To get a lifelike replica, or something close to it, of a loved one just before that loved one passed away, cost a lot of money. So, if Romans had enough money and status, they could commission an artist to render that image which they could use to not only honor the loved one but also to remember what that person looked like much like how a photograph is used today. Even Romans with less wealth and